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1994-10-25
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Document 2693
DOCN M94A2693
TI Evaluation of a rapid and simple HIV-1 assay.
DT 9412
AU Zhang X; Constantine NT; Dept. Pathology, Univ. of Maryland Sch. of
Med., Balto.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):246 (abstract no. PB0413). Unique
Identifier : AIDSLINE ICA10/94369882
AB OBJECTIVES: To evaluate the performance of the Health Test HIV-1 rapid
assay when testing sera from inner-city hospital patients in the US, and
to determine concordance with a routine FDA-licensed ELISA. METHODS: 850
serum samples were collected from sequential cases received at the
Clinical Immunology Laboratory at the University of Maryland Medical
System/Hospital in Baltimore. All samples were centrifuged, stored at 4
degrees C, and tested using an HIV-1/2 ELISA (Genetic Systems) and the
Health Test HIV-1 assay (Akers Laboratories, USA) as recommended by the
manufacturers. Any samples yielding a reactive result were retested by
the respective test and confirmed by Western blot (BioRad). Samples
which exhibited discrepant results were retested by all assays to ensure
that technical error had not occurred. RESULTS: Of the 850 sera, 56
(6.6%) produced reactive results by both tests and were confirmed by
Western blot (WB). There were 11 false positive results and one positive
sample which was difficult to read by the Health Test as compared with
ELISA and WB. Except for the one sample that was difficult to interpret,
the sensitivity was 100%, and the specificity was 98.8%. DISCUSSION AND
CONCLUSIONS: The Health Test HIV-1 assay is a very simple, rapid, and
economical test for detecting HIV antibodies. It requires only one
minute for completion and the test strips can be kept for permanent
records. We are currently investigating the cause of the one sample
which was difficult to interpret.
DE AIDS Serodiagnosis/*METHODS Baltimore Blotting, Western Comparative
Study Enzyme-Linked Immunosorbent Assay/*METHODS Human HIV
Antibodies/*BLOOD HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*IMMUNOLOGY Predictive Value of Tests *Urban Population MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).